SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Trott,G.; Pereira-Lima,J.F.S.; Leães,C.G.S.; Ferreira,N.P.; Barbosa-Coutinho,L.M.; Oliveira,M.C..

Meningiomas are common, usually benign tumors, with a high postoperative recurrence rate. However, the genesis and development of these tumors remain controversial. We aimed to investigate the presence and implications of a mutated p53 protein and dopamine D2 receptor in a representative series of meningiomas and to correlate these findings with age, gender, tumor grade, and recurrence. Tumor tissue samples of 157 patients diagnosed with meningioma (37 males and 120 females, mean age 53.6±14.3 years) who underwent surgical resection between 2003 and 2012 at our institution were immunohistochemically evaluated for the presence of p53 protein and dopamine D2 receptor and were followed-up to analyze tumor recurrence or regrowth. Tumors were classified as...

Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients

Palavras-chave: Receptors; Dopamine D2; Immunohistochemistry; Meningioma; Tumor suppressor protein p53; Recurrence.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500415

Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan.

Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by...

Accuracy of dose planning for prostate radiotherapy in the presence of metallic implants evaluated by electron spin resonance dosimetry

Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606

Alves,G.G.; Kinoshita,A.; Oliveira,H.F. de; Guimarães,F.S.; Amaral,L.L.; Baffa,O..

Radiotherapy is one of the main approaches to cure prostate cancer, and its success depends on the accuracy of dose planning. A complicating factor is the presence of a metallic prosthesis in the femur and pelvis, which is becoming more common in elderly populations. The goal of this work was to perform dose measurements to check the accuracy of radiotherapy treatment planning under these complicated conditions. To accomplish this, a scale phantom of an adult pelvic region was used with alanine dosimeters inserted in the prostate region. This phantom was irradiated according to the planned treatment under the following three conditions: with two metallic prostheses in the region of the femur head, with only one prosthesis, and without any prostheses. The...

Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

Palavras-chave: Alanine electron spin resonance dosimetry; Prostate; Electron paramagnetic resonance; Treatment-planning system; Inhomogeneity correction.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700644

Diaz,R.S.; Sabino,E.C..

For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation. The development of enzymes with an inherent 3' to 5' exonuclease proofreading activity, lacking in Taq polymerase, would be an improvement when higher fidelity is needed. We used the forward mutation assay to compare the fidelity of Taq polymerase and Thermotoga maritima (ULTMA) DNA polymerase, an enzyme that does have proofreading activity. We did not find significant differences in the fidelity of either enzyme, even when using optimal...

Acetylcholine and bradykinin enhance hypotension and affect the function of remodeled conduit arteries in SHR and SHR treated with nitric oxide donors

Palavras-chave: Polymerase chain reaction fidelity; Thermus aquaticus (Taq) DNA polymerase; Thermotoga maritima DNA polymerase.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000001

Gerová,M.; Kristek,F.; Cacányiová,S.; Cebová,M..

Discrepancy was found between enhanced hypotension and attenuated relaxation of conduit arteries in response to acetylcholine (ACh) and bradykinin (BK) in nitric oxide (NO)-deficient hypertension. The question is whether a similar phenomenon occurs in spontaneously hypertensive rats (SHR) with a different pathogenesis. Wistar rats, SHR, and SHR treated with NO donors [molsidomine (50 mg/kg) or pentaerythritol tetranitrate (100 mg/kg), twice a day, by gavage] were studied. After 6 weeks of treatment systolic blood pressure (BP) was increased significantly in experimental groups. Under anesthesia, the carotid artery was cannulated for BP recording and the jugular vein for drug administration. The iliac artery was used for in vitro studies and determination...

Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock

Palavras-chave: Hypertension; Conduit iliac artery; Resistance arteries; Molsidomine; Pentaerythritol tetranitrate; Iliac artery; Structure.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600019

Camarini,R.; Benedito,M.A.C..

An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem...

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney

Palavras-chave: Acetylcholinesterase; Medulla oblongata; Pons; Chronic electroconvulsive shock.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000012

Monteiro,H.S.A.; Silva,I.M.S.C. da; Martins,A.M.C.; Fonteles,M.C..

Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g% of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport (%TNa+). The infusion of Crotalus durissus terrificus venom (10 µg/ml) and crotoxin (10 µg/ml) increased GFR...

Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae)

Palavras-chave: Crotalus durissus terrificus venom; Crotoxin; Kidney.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000017

Sartorello,R.; Garcia,C.R.S..

An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER) and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge...

Activation of endogenous angiotensin converting enzyme 2 prevents early injuries induced by hyperglycemia in rat retina

Palavras-chave: Red blood cells; Calcium; Teiidae; Ameiva ameiva; Purinoceptor; UTP.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100002

Foureaux,G.; Nogueira,B. S.; Coutinho,D. C. O.; Raizada,M. K.; Nogueira,J. C.; Ferreira,A. J..

Diabetic retinopathy (DR) is a serious complication of diabetes mellitus that may result in blindness. We evaluated the effects of activation of endogenous angiotensin converting enzyme (ACE) 2 on the early stages of DR. Rats were administered an intravenous injection of streptozotocin to induce hyperglycemia. The ACE2 activator 1-[[2-(dimethylamino) ethyl] amino]-4-(hydroxymethyl)-7-[[(4-methylphenyl) sulfonyl] oxy]-9H-xanthone 9 (XNT) was administered by daily gavage. The death of retinal ganglion cells (RGC) was evaluated in histological sections, and retinal ACE2, caspase-3, and vascular endothelial growth factor (VEGF) expressions were analyzed by immunohistochemistry. XNT treatment increased ACE2 expression in retinas of hyperglycemic (HG) rats...

Activation of locus coeruleus heme oxygenase-carbon monoxide pathway promoted an anxiolytic-like effect in rats

Palavras-chave: ACE2 activation; Angiotensin-(1-7); Renin-angiotensin system; Eyes; Diabetic retinopathy.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201109

Carvalho-Costa,P.G.; Branco,L.G.S.; Leite-Panissi,C.R.A..

The heme oxygenase-carbon monoxide pathway has been shown to play an important role in many physiological processes and is capable of altering nociception modulation in the nervous system by stimulating soluble guanylate cyclase (sGC). In the central nervous system, the locus coeruleus (LC) is known to be a region that expresses the heme oxygenase enzyme (HO), which catalyzes the metabolism of heme to carbon monoxide (CO). Additionally, several lines of evidence have suggested that the LC can be involved in the modulation of emotional states such as fear and anxiety. The purpose of this investigation was to evaluate the activation of the heme oxygenase-carbon monoxide pathway in the LC in the modulation of anxiety by using the elevated plus maze test (EPM)...

Activation of neural cholecystokinin-1 receptors induces relaxation of the isolated rat duodenum which is reduced by nitric oxide synthase inhibitors

Palavras-chave: Heme oxygenase Carbon monoxide Locus coeruleus Anxiety Elevated plus maze; Light/dark box.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500601

Martins,S.R.; de Oliveira,R.B.; Ballejo,G..

Cholecystokinin (CCK) influences gastrointestinal motility, by acting on central and peripheral receptors. The aim of the present study was to determine whether CCK has any effect on isolated duodenum longitudinal muscle activity and to characterize the mechanisms involved. Isolated segments of the rat proximal duodenum were mounted for the recording of isometric contractions of longitudinal muscle in the presence of atropine and guanethidine. CCK-8S (EC50: 39; 95% CI: 4.1-152 nM) and cerulein (EC50: 58; 95% CI: 18-281 nM) induced a concentration-dependent and tetrodotoxin-sensitive relaxation. Nomeganitro-L-arginine (L-NOARG) reduced CCK-8S- and cerulein-induced relaxation (IC50: 5.2; 95% CI: 2.5-18 µM) in a concentration-dependent manner. The magnitude...

Activity and functional interaction of alternative oxidase and uncoupling protein in mitochondria from tomato fruit

Palavras-chave: CCK; CCK receptor subtype 1; Proximal duodenum; Myenteric neurons; Nitric oxide.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200014

Sluse,F.E.; Jarmuszkiewicz,W..

Cyanide-resistant alternative oxidase (AOX) is not limited to plant mitochondria and is widespread among several types of protists. The uncoupling protein (UCP) is much more widespread than previously believed, not only in tissues of higher animals but also in plants and in an amoeboid protozoan. The redox energy-dissipating pathway (AOX) and the proton electrochemical gradient energy-dissipating pathway (UCP) lead to the same final effect, i.e., a decrease in ATP synthesis and an increase in heat production. Studies with green tomato fruit mitochondria show that both proteins are present simultaneously in the membrane. This raises the question of a specific physiological role for each energy-dissipating system and of a possible functional connection...

Activity of liver microsomal enzymes during the chronic phase of murine schistosomiasis

Palavras-chave: Plant mitochondria; Alternative oxidase; Uncoupling protein; Regulation; Electron partitioning.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300002

Conte,F.P.; Fidalgo-Neto,A.A.; Manhães-Rocha,D.A.; Paumgartten,F.J.R.; De-Oliveira,A.C.A.X..

The effects of schistosomiasis on microsomal enzymes were studied on post-infection day 90 when accumulated damage and fibrosis are most intense but granulomatous reaction around the eggs harbored in the liver is smaller than during the earlier phases. Swiss Webster (SW) and DBA/2 mice of either sex (N = 12 per sex per group) were infected with 100 Schistosoma mansoni cercariae on postnatal day 10 and killed on post-infection day 90. Cytochrome P-450 (CYP) concentration and alkoxyresorufin-O-dealkylases (EROD, MROD, BROD, and PROD), p-nitrophenol-hydroxylase (PNPH), coumarin-7-hydroxylase (COH), and UDP-glucuronosyltransferase (UGT) activities were measured in hepatic microsomes. Age-matched mice of the same sex and strain were used as controls. In S....

Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity

Palavras-chave: Schistosoma mansoni; Pharmacokinetics; Cytochrome P-450; Monooxygenases; Granuloma; Liver microsomes.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500008

Rodríguez,E.M.; Bigi,R.; Medesani,D.A.; Stella,V.S.; Greco,L.S.L.; Moreno,P.A.R.; Monserrat,J.M.; Pellerano,G.N.; Ansaldo,M..

Whole body oxygen consumption and some hemolymph parameters such as pH, partial pressure of gases, level of ions and lactate were measured in the estuarine crab Chasmagnathus granulata after both acute (96 h) and chronic (2 weeks) exposure to cadmium at concentrations ranging from 0.4 to 6.3 mg/l. In all instances, the crabs developed hemolymph acidosis, but no respiratory (increased PCO2) or lactate increases were evident. Hemolymph levels of sodium and calcium were always increased by cadmium exposure. The chronic toxicity of cadmium was enhanced at 12 salinity, even causing a significantly higher mortality in comparison with the higher salinity (30) used. A general metabolic arrest took place at 12 salinity in the crabs chronically exposed to...

Acute and chronic electrical activation of baroreceptor afferents in awake and anesthetized subjects

Palavras-chave: Cadmium; Crabs; Blood homeostasis; Salinity.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400011

Durand,M.T.; Fazan Jr.,R.; Salgado,M.C.O.; Salgado,H.C..

Electrical stimulation of baroreceptor afferents was used in the 1960's in several species, including human beings, for the treatment of refractory hypertension. This approach bypasses the site of baroreceptor mechanosensory transduction. Chronic electrical stimulation of arterial baroreceptors, particularly of the carotid sinus nerve (Hering's nerve), was proposed as an ultimate effort to treat refractory hypertension and angina pectoris due to the limited nature of pharmacological therapy available at that time. Nevertheless, this approach was abandoned in the early 1970's due to technical limitations of implantable devices and to the development of better-tolerated antihypertensive medications. More recently, our laboratory developed the technique of...

Acute AT1 receptor blockade does not improve the depressed baroreflex in rats with chronic renal hypertension

Palavras-chave: Carotid sinus nerve; Aortic depressor nerve; Hemodynamics; Baroreceptor stimulation; Refractory hypertension.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100009

Farah,V.M.A.; Moreira,E.D.; Ushizima,M.; Cestari,I.A.; Irigoyen,M.C.; Krieger,E.M..

To assess the role of angiotensin II in the sensitivity of the baroreflex control of heart rate (HR) in normotensive rats (N = 6) and chronically hypertensive rats (1K1C, 2 months, N = 7), reflex changes of HR were evaluated before and after (15 min) the administration of a selective angiotensin II receptor antagonist (losartan, 10 mg/kg, iv). Baseline values of mean arterial pressure (MAP) were higher in hypertensive rats (195 ± 6 mmHg) than in normotensive rats (110 ± 2 mmHg). Losartan administration promoted a decrease in MAP only in hypertensive rats (16%), with no changes in HR. During the control period, the sensitivity of the bradycardic and tachycardic responses to acute MAP changes were depressed in hypertensive rats (~70% and ~65%, respectively)...

Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats

Palavras-chave: Baroreflex sensitivity; Losartan; Angiotensin II; Renal hypertension.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200014

de-Oliveira,G.R.; Gondim,F. de-A.A.; da-Graça,J.R.V.; Xavier-Neto,J.; Dantas,R.P.; Gondim,R.B.M.; Santos,A.A.; Rola,F.H..

The present study evaluates the effect of blood volume expansion on the gastrointestinal transit of a charchoal meal (2.5 ml of an aqueous suspension consisting of 5% charcoal and 5% gum arabic) in awake male Wistar rats (200-270 g). On the day before the experiments, the rats were anesthetized with ether, submitted to left jugular vein cannulation and fasted with water ad libitum until 2 h before the gastrointestinal transit measurement. Blood volume expansion by iv infusion of 1 ml/min Ringer bicarbonate in volumes of 3, 4 or 5% body weight delayed gastrointestinal transit at 10 min after test meal administration by 21.3-26.7% (P<0.05), but no effect was observed after 1 or 2% body weight expansion. The effect of blood volume expansion (up to 5%...

Acute buspirone abolishes the expression of behavioral dopaminergic supersensitivity in mice

Palavras-chave: Hypervolemia; Blood volume; Gastrointestinal transit; Rats; Gastrointestinal motility.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017

Queiroz,C.M.T.; Alcântara,F.B.; Yagüe,A.M.L.; Bibancos,T.; Frussa-Filho,R..

Previous studies have shown that rats withdrawn from long-term treatment with dopamine receptor blockers exhibit dopaminergic supersensitivity, which can be behaviorally evaluated by enhanced general activity observed in an open-field. Recently, it has been reported that co-treatment with the non-benzodiazepine anxiolytic buspirone attenuates the development of haloperidol-induced dopaminergic supersensitivity measured by open-field behavior of rats. The aims of the present study were: 1) to determine, as previously reported for rats, if mice withdrawn from long-term neuroleptic treatment would also develop dopaminergic supersensitivity using open-field behavior as an experimental paradigm, and 2) to examine if acute buspirone administration would...

Acute but not chronic metabolic acidosis potentiates the acetylcholine-induced reduction in blood pressure: an endothelium-dependent effect

Palavras-chave: Buspirone; Haloperidol; Dopaminergic supersensitivity; Tardive dyskinesia; Behavior; Mice.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200013

Celotto,A.C.; Ferreira,L.G.; Capellini,V.K.; Albuquerque,A.A.S.; Rodrigues,A.J.; Evora,P.R.B..

Metabolic acidosis has profound effects on vascular tone. This study investigated the in vivo effects of acute metabolic acidosis (AMA) and chronic metabolic acidosis (CMA) on hemodynamic parameters and endothelial function. CMA was induced by ad libitum intake of 1% NH4Cl for 7 days, and AMA was induced by a 3-h infusion of 6 M NH4Cl (1 mL/kg, diluted 1:10). Phenylephrine (Phe) and acetylcholine (Ach) dose-response curves were performed by venous infusion with simultaneous venous and arterial blood pressure monitoring. Plasma nitrite/nitrate (NOx) was measured by chemiluminescence. The CMA group had a blood pH of 7.15±0.03, which was associated with reduced bicarbonate (13.8±0.98 mmol/L) and no change in the partial pressure of arterial carbon dioxide...

Acute effect of alcohol intake on sine-wave Cartesian and polar contrast sensitivity functions

Palavras-chave: Acid-base balance; Metabolic acidosis; Hemodynamic failure; Endothelium; Nitric oxide.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200604

Cavalcanti-Galdino,M.K.; da Silva,J.A.; Mendes,L.C.; dos Santos,N.A.; Simas,M.L.B..

The aim of this study was to assess contrast sensitivity for angular frequency stimuli as well as for sine-wave gratings in adults under the effect of acute ingestion of alcohol. We measured the contrast sensitivity function (CSF) for gratings of 0.25, 1.25, 2.5, 4, 10, and 20 cycles per degree of visual angle (cpd) as well as for angular frequency stimuli of 1, 2, 4, 24, 48, and 96 cycles/360°. Twenty adults free of ocular diseases, with normal or corrected-to-normal visual acuity, and no history of alcoholism were enrolled in two experimental groups: 1) no alcohol intake (control group) and 2) alcohol ingestion (experimental group). The average concentration of alcohol in the experimental group was set to about 0.08%. We used a paradigm involving a...